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Inhibition of LPS-induced <t>TNF-</t> α (pg/ml) production by HNK, MCP, and MCP : HNK (9 : 1) in RAW 264.7 mouse monocyte cell line. The cells were treated with compounds and/or LPS in starvation medium and <t>TNF-</t> <t>α</t> analyzed by <t>ELISA.</t> Inhibition curves were analyzed by paired t test; ∗ p < 0.05 for HNK versus MCP, and HNK versus MCP : HNK (9 : 1); S, synergism between MCP and HNK.
Mouse Tnf α Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Inhibition of LPS-induced <t>TNF-</t> α (pg/ml) production by HNK, MCP, and MCP : HNK (9 : 1) in RAW 264.7 mouse monocyte cell line. The cells were treated with compounds and/or LPS in starvation medium and <t>TNF-</t> <t>α</t> analyzed by <t>ELISA.</t> Inhibition curves were analyzed by paired t test; ∗ p < 0.05 for HNK versus MCP, and HNK versus MCP : HNK (9 : 1); S, synergism between MCP and HNK.
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Inhibition of LPS-induced <t>TNF-</t> α (pg/ml) production by HNK, MCP, and MCP : HNK (9 : 1) in RAW 264.7 mouse monocyte cell line. The cells were treated with compounds and/or LPS in starvation medium and <t>TNF-</t> <t>α</t> analyzed by <t>ELISA.</t> Inhibition curves were analyzed by paired t test; ∗ p < 0.05 for HNK versus MCP, and HNK versus MCP : HNK (9 : 1); S, synergism between MCP and HNK.
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tnf α  (R&D Systems)
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R&D Systems tnf α
Inhibition of LPS-induced <t>TNF-</t> α (pg/ml) production by HNK, MCP, and MCP : HNK (9 : 1) in RAW 264.7 mouse monocyte cell line. The cells were treated with compounds and/or LPS in starvation medium and <t>TNF-</t> <t>α</t> analyzed by <t>ELISA.</t> Inhibition curves were analyzed by paired t test; ∗ p < 0.05 for HNK versus MCP, and HNK versus MCP : HNK (9 : 1); S, synergism between MCP and HNK.
Tnf α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TcpF suppresses <t>TNF-</t> α induction. (a) E. faecalis Symbioflor 1 and its isogenic tcpF deletion mutant Symbioflor 1 Δ tcpF show identical growth rate. Fresh culture was started from overnight culture 1 : 25 in BHI at 37°C and 100 rpm. Optical density at 600 nm (OD 600 ) at indicated time points. (b) RAW264.7 macrophages were infected for 6 h with E. faecalis Symbioflor 1 and E . faecalis Symbioflor 1 Δ tcpF at indicated moi. NC: negative control—uninfected macrophages. Error bars represent s.d. ( n = 4). Student's t -test: ∗∗∗ P < 0,001; ∗∗ P < 0,01.
Mouse Tnf α Quantikine Elisa Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TcpF suppresses <t>TNF-</t> α induction. (a) E. faecalis Symbioflor 1 and its isogenic tcpF deletion mutant Symbioflor 1 Δ tcpF show identical growth rate. Fresh culture was started from overnight culture 1 : 25 in BHI at 37°C and 100 rpm. Optical density at 600 nm (OD 600 ) at indicated time points. (b) RAW264.7 macrophages were infected for 6 h with E. faecalis Symbioflor 1 and E . faecalis Symbioflor 1 Δ tcpF at indicated moi. NC: negative control—uninfected macrophages. Error bars represent s.d. ( n = 4). Student's t -test: ∗∗∗ P < 0,001; ∗∗ P < 0,01.
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mouse cxcl2/mip-2 quantikine elisa kit  (Bio-Techne corporation)
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Bio-Techne corporation mouse cxcl2/mip-2 quantikine elisa kit
TcpF suppresses <t>TNF-</t> α induction. (a) E. faecalis Symbioflor 1 and its isogenic tcpF deletion mutant Symbioflor 1 Δ tcpF show identical growth rate. Fresh culture was started from overnight culture 1 : 25 in BHI at 37°C and 100 rpm. Optical density at 600 nm (OD 600 ) at indicated time points. (b) RAW264.7 macrophages were infected for 6 h with E. faecalis Symbioflor 1 and E . faecalis Symbioflor 1 Δ tcpF at indicated moi. NC: negative control—uninfected macrophages. Error bars represent s.d. ( n = 4). Student's t -test: ∗∗∗ P < 0,001; ∗∗ P < 0,01.
Mouse Cxcl2/Mip 2 Quantikine Elisa Kit, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse cxcl12/sdf-1 alpha quantikine elisa kit  (Bio-Techne corporation)
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Bio-Techne corporation mouse cxcl12/sdf-1 alpha quantikine elisa kit
TcpF suppresses <t>TNF-</t> α induction. (a) E. faecalis Symbioflor 1 and its isogenic tcpF deletion mutant Symbioflor 1 Δ tcpF show identical growth rate. Fresh culture was started from overnight culture 1 : 25 in BHI at 37°C and 100 rpm. Optical density at 600 nm (OD 600 ) at indicated time points. (b) RAW264.7 macrophages were infected for 6 h with E. faecalis Symbioflor 1 and E . faecalis Symbioflor 1 Δ tcpF at indicated moi. NC: negative control—uninfected macrophages. Error bars represent s.d. ( n = 4). Student's t -test: ∗∗∗ P < 0,001; ∗∗ P < 0,01.
Mouse Cxcl12/Sdf 1 Alpha Quantikine Elisa Kit, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Inhibition of LPS-induced TNF- α (pg/ml) production by HNK, MCP, and MCP : HNK (9 : 1) in RAW 264.7 mouse monocyte cell line. The cells were treated with compounds and/or LPS in starvation medium and TNF- α analyzed by ELISA. Inhibition curves were analyzed by paired t test; ∗ p < 0.05 for HNK versus MCP, and HNK versus MCP : HNK (9 : 1); S, synergism between MCP and HNK.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Synergistic Antioxidant and Anti-Inflammatory Effects between Modified Citrus Pectin and Honokiol

doi: 10.1155/2017/8379843

Figure Lengend Snippet: Inhibition of LPS-induced TNF- α (pg/ml) production by HNK, MCP, and MCP : HNK (9 : 1) in RAW 264.7 mouse monocyte cell line. The cells were treated with compounds and/or LPS in starvation medium and TNF- α analyzed by ELISA. Inhibition curves were analyzed by paired t test; ∗ p < 0.05 for HNK versus MCP, and HNK versus MCP : HNK (9 : 1); S, synergism between MCP and HNK.

Article Snippet: TNF- α produced and secreted into the medium by the cells was analyzed by ELISA protocol using the mouse TNF- α Quantikine ELISA kit (R&D systems, Minneapolis, MN) as per manufacturer's instructions [ ].

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay

Dose-effect relationship between modified citrus pectin (MCP) and honokiol (HNK).

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Synergistic Antioxidant and Anti-Inflammatory Effects between Modified Citrus Pectin and Honokiol

doi: 10.1155/2017/8379843

Figure Lengend Snippet: Dose-effect relationship between modified citrus pectin (MCP) and honokiol (HNK).

Article Snippet: TNF- α produced and secreted into the medium by the cells was analyzed by ELISA protocol using the mouse TNF- α Quantikine ELISA kit (R&D systems, Minneapolis, MN) as per manufacturer's instructions [ ].

Techniques: Modification, Inhibition, Antioxidant Activity Assay, Activity Assay

TcpF suppresses TNF- α induction. (a) E. faecalis Symbioflor 1 and its isogenic tcpF deletion mutant Symbioflor 1 Δ tcpF show identical growth rate. Fresh culture was started from overnight culture 1 : 25 in BHI at 37°C and 100 rpm. Optical density at 600 nm (OD 600 ) at indicated time points. (b) RAW264.7 macrophages were infected for 6 h with E. faecalis Symbioflor 1 and E . faecalis Symbioflor 1 Δ tcpF at indicated moi. NC: negative control—uninfected macrophages. Error bars represent s.d. ( n = 4). Student's t -test: ∗∗∗ P < 0,001; ∗∗ P < 0,01.

Journal: International Journal of Microbiology

Article Title: The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response

doi: 10.1155/2014/918143

Figure Lengend Snippet: TcpF suppresses TNF- α induction. (a) E. faecalis Symbioflor 1 and its isogenic tcpF deletion mutant Symbioflor 1 Δ tcpF show identical growth rate. Fresh culture was started from overnight culture 1 : 25 in BHI at 37°C and 100 rpm. Optical density at 600 nm (OD 600 ) at indicated time points. (b) RAW264.7 macrophages were infected for 6 h with E. faecalis Symbioflor 1 and E . faecalis Symbioflor 1 Δ tcpF at indicated moi. NC: negative control—uninfected macrophages. Error bars represent s.d. ( n = 4). Student's t -test: ∗∗∗ P < 0,001; ∗∗ P < 0,01.

Article Snippet: The secretion of TNF- α was measured using the mouse TNF- α Quantikine ELISA kit (BD) on a Phomo Plate reader (Anthos Mikrosystme) according to the kits manual.

Techniques: Mutagenesis, Infection, Negative Control